ผลต่างระหว่างรุ่นของ "หน้าหลัก"

รุ่นแก้ไขเมื่อ 07:15, 22 ธันวาคม 2564

Varieties of Koi Fishes Koi keeping is enjoyed by not just the Japanese people but practically every one of the races on the planet. That is no wonder considering that the beauty of these fish is one of simplicity and loveliness. No matter how hard it really is to keep a Koi pond, fish keepers are generally more than willing for making and their very own. It is considered the fist various Koi fish comprises of three, the most famous of which will be the Kohaku. Kuhakos are those which may have white bodies with red markings. They are once grown for food purposes only then again the discovery of their decoration purposes made them much more popular. After several years later too, by making use of the ingenuity of Koi breeders, there are now numerous fish varieties. Koi varieties are ample yet you can't imagine to possess every one of them a single purchase. This is exactly exactly why keepers of these kinds of fish have kept the fire alive because of the knowledge they've still much to discover. In keeping a fish pond, having different varieties of such fishes swimming everywhere in the area is one area that can cause an awesome and natural effect to one's senses. You will always be confident to demonstrate to your guests your pond. The Kuhako Koi for just one consists of many varieties. Their differences mainly will lie upon the several patterns with their markings. There is a variety the location where the red markings look like lighting as there is one which has only one red mark on its fancy goldfish for sale forehead. Taisho-sanke Koi an added other hand have red and black markings into it back while Bekko Koi is made up of Koi fishes who have yellow, white or red wine bodies with black markings. Other Koi varieties also include Gin Rin, Kawarimo, Hikari Utsuri and Butterfly Koi. Each variety possesses his own outstanding feature. You can find them generally in most Koi breeders and fish keepers who practice breeding of such Fishes.

ผลต่างระหว่างรุ่นของ "หน้าหลัก"

รุ่นแก้ไขเมื่อ 07:15, 22 ธันวาคม 2564

รายการเลือกการนำทาง

เครื่องมือส่วนตัว

เนมสเปซ

สิ่งที่แตกต่าง

ดู

เพิ่มเติม

ค้นหา

การนำทาง

เครื่องมือ

@@ แถว 1: / แถว 1: @@
-The empty expression vector was established as the control. Removal of Dox in the medium induced the expression of FLAG.SCUBE2-FL, -ty97, and -D4 protein, as confirmed by anti-FLAG Western blot analysis (Fig. 1B). However, SCUBE2-FL or mutant protein was not induced in the presence of Dox in these stable MCF-7 cell lines or in the control MCF-7 Tet-Off vector clone (Fig. 1B). Overexpression of SCUBE2 or Its Mutant Proteins Suppresses Proliferation, but Does Not Increase Apoptosis, of MCF-7 Breast Cancer Cells Both in Vitro and in Vivo--To investigate the effects of SCUBE2 expression on breast cancer cell growth, the MCF-7 Tet-Off vector and SCUBE2-FL, ty97, and -D4 stable cells were cultured in the presence or absence of Dox for up to 6 days to suppress or induce the expression of ectopic FLAG. SCUBE2-FL, -ty97, or -D4 protein. Cell growth was then measured by MTT assay. Induction of ectopic SCUBE2-FL protein,doxycycline (( )Dox) or not (( )Dox). Growth of the MCF-7 Tet-Off vector or SCUBE2-ty97 or -D4 cells was measured as a function of time in the absence of doxycycline. Data points, mean tumor volumes (length width height 0.5236 (in mm3)); error bars, S.E. *, p 0.01 (n 10 or 7 for the MCF-7 Tet-Off vector tumors, n 10 for the SCUBE2-ty97 tumors, and n 7 for the SCUBE2-D4 tumors).JULY 29, 2011 ?VOLUME 286 ?NUMBERJOURNAL OF BIOLOGICAL CHEMISTRYMolecular Analysis of Breast Tumor Suppressor Gene SCUBEFIGURE 3. SCUBE2 antagonizes autocrine BMP-mediated signaling through its CUB domain. MCF-7 Tet-Off vector and SCUBE2-FL, -ty97, and -D4 stable cells were cultured in medium without doxycycline (( )Dox) for 5 days, and the induction of ectopic NH2-terminal FLAG-tagged SCUBE2-FL or deletion mutant protein (ty97 or D4) was determined by Western blot (WB) analysis with anti-FLAG antibody (bottom panel). Total protein lysates (20 g) from each cell line in duplicate were subjected to Western blot analysis with an antibody specific against the phosphorylated Smad1/5/8 (p-Smad1/5/8) or total Smad1 protein (top panel and second panel). Ponceau red staining of the PVDF membrane confirmed the relative loading of total proteins for each sample (third panel). Shown is a histographic representation of quantified data of the phosphorylated Smad1/5/8 and total Smad1 protein concentrations in respective groups (quantified by densitometric scanning and normalized by the total amount of protein loading). Data are means S.E. of two experiments. *, p 0.01 compare with ( )Dox.as well as SCUBE2-ty97 and -D4, suppressed the proliferation of these MCF-7 cell lines in the absence of Dox (Fig. 2A). The control MCF-7 Tet-Off vector clone and SCUBE-FL, -ty97, and -D4 cells did not differ in growth on culture with Dox to block the expression of ectopic SCUBE2 proteins (data not shown). Because overexpression of SCUBE2-ty97 or -D4 protein inhibited MCF-7 breast cancer cell growth in vitro, we next examined breast tumor growth in vivo in nude mice. MCF-7 Tet-Off vector or SCUBE2-ty97 or -D4 cells were injected into the mammary fat pads of nude mice that received estrogen pellets to promote the growth and development of breast tumors.
+Varieties of Koi Fishes
+Koi keeping is enjoyed by not just the Japanese people but practically every one of the races on the planet. That is no wonder considering that the beauty of these fish is one of simplicity and loveliness. No matter how hard it really is to keep a Koi pond, fish keepers are generally more than willing for making and their very own.
+It is considered the fist various Koi fish comprises of three, the most famous of which will be the Kohaku. Kuhakos are those which may have white bodies with red markings. They are once grown for food purposes only then again the discovery of their decoration purposes made them much more popular. After several years later too, by making use of the ingenuity of Koi breeders, there are now numerous fish varieties.
+Koi varieties are ample yet you can't imagine to possess every one of them a single purchase. This is exactly exactly why keepers of these kinds of fish have kept the fire alive because of the knowledge they've still much to discover. In keeping a fish pond, having different varieties of such fishes swimming everywhere in the area is one area that can cause an awesome and natural effect to one's senses. You will always be confident to demonstrate to your guests your pond.
+The Kuhako Koi for just one consists of many varieties. Their differences mainly will lie upon the several patterns with their markings. There is a variety the location where the red markings look like lighting as there is one which has only one red mark on its [https://www.windsorfishhatchery.com.au/ fancy goldfish for sale] forehead. Taisho-sanke Koi an added other hand have red and black markings into it back while Bekko Koi is made up of Koi fishes who have yellow, white or red wine bodies with black markings. Other Koi varieties also include Gin Rin, Kawarimo, Hikari Utsuri and Butterfly Koi. Each variety possesses his own outstanding feature. You can find them generally in most Koi breeders and fish keepers who practice breeding of such Fishes.