ผลต่างระหว่างรุ่นของ "หน้าหลัก"

จาก wiki.surinsanghasociety
ไปยังการนำทาง ไปยังการค้นหา
ล
ล
แถว 1: แถว 1:
−
YAR066w (TFP1) can be a hypothetical ORF containing 23 aa of a putative signal sequence as well as a glycosylphosphatidyl inositol (GPI)-anchor motif. ULI1 (TFP2) encodes a putative protein of unknown function induced by the ER unfolded protein response and consists of 19 aa of a putative signal sequence. CIS3 (TFP3), a cik1 suppressor with internal repeats, encodes a cell wall protein with 21 aa of a putative pre-signal and 43 aa of a pro-sequence processed by Kex2p24.Trapping of optimal TFPs from a genomic DNA library for secretion of hIL-2, a hardly ever secreted protein in yeast. Secretion of invertase, encoded by SUC2, is prerequisite for yeast cell development onIdentification of an essential domain of TFP1 for protein secretion. To make sure secretion of hIL-2 applying the screened TFPs without invertase, the SUC2 gene of each vector was removed, and also the yeast dipeptidyl protease Kex2p25 cleavage internet site (LDKR) was inserted among TFP and hIL2 by PCR. Amongst three tested TFPs, TFP1 was superior to the other TFPs for hIL-2 secretion (information not shown). The TFP1 nucleotide sequence contained five domains that could possibly be important for secretion of hIL-2 (domain A: signal sequence, B: N-glycosylation web-site, C: Ser- and Ala-rich sequence, D: a flanking sequence, in addition to a 5 -untranslated region [UTR]). Four truncated TFP1 derivatives have been constructed to recognize the effects of every single domain on hIL-2 secretion (Fig.  2a), along with the culture supernatants of every single construct had been analysed by SDS-PAGE (Fig. 2b). The hIL-2 protein fused with TFP1-1 and TFP1-2, containing the signal sequence or N-glycosylation web site only, was not secreted or perhaps detected by western blot analysis, but was detected within the intracellular fraction of every cell (Fig. 2c). The slightly bigger size of those proteins compared together with the secreted hIL-2 indicated that they had been not processed by Kex2p. Therefore, these fusion proteins might not reach the Golgi complicated and will be caught by the ER good [https://www.medchemexpress.com mce custom synthesis] quality control technique.Scientific RepoRts | five:12229 | DOi: ten.1038/srepwww.nature.com/scientificreports/Figure 1. Invertase-based translational fusion partner trap technique. (a) Development patterns of Saccharomyces cerevisiae Y2805 suc2 recombinant strains carrying the indicated plasmids on diverse carbon sources, glucose and sucrose. (b) Schematic representation from the genetic screening technique for the cloning of optimal translational fusion partners (TFPs) to accelerate secretion of target proteins (X).This result clearly showed that hIL-2 was not secreted by a standard strategy employing only a secretion signal. TFP1-3, containing domains A, B, and C, secreted authentic hIL-2 using a tiny level of unprocessed and degraded protein, but still significantly less than that with the original TFP1 (Fig. 2b), suggesting that all TFP1 domains had been necessary for hIL-2 secretion. Ultimately, hIL-2 secretion was augmented by about three-fold right after removing the five -UTR (TFP1?) (Fig. 2b). Some yeast genes are known to contain unfavorable cis-acting components that have an effect on the translational efficiency around the 5 -UTR26.The protein is revealed by Coomassie staining. (c) Western blot analysis of intracellular and extracellular proteins made by recombinant strains carrying the indicated plasmids. C: host strain carrying the mock vector.
+
Nd Shekhertek villages in Rangpur district [79]; discomfort in body, blood purifier
 +
Nd Shekhertek villages in Rangpur district [79]; discomfort in physique, blood purifier by FMPs of Terbaria and Babla villages in Tangail district [81]; pain by TMPs of Bongshi tribe in Tangail district [82]; malaria fever, any form of stomach discomfort by TMPs of Tonchongya tribe of Bandarban district [82]; feeling of weakness in the course of time of menstruation by TMPs on the Harbang clan from the Tripura tribe of Mirsharai area, Chittagong district [95]; fever in kids, toothache, discomfort in gums by FMPs of Dhamrai sub-district, Dhaka district [97]; hookworm infection by FMPs of Barisal Town, Barisal district [98]; stomach pain by TMPs of Chakma tribe of Rangapanir Chara Area in Khagrachaari district [34]; jaundice, helminthiasis by a TMP in the Deb barma clan on the Tripura tribe of Moulvibazar district [103]; jaundice by a FMP of Sreemangal Upazila in Maulvibazar district [105]; skin eruption, fever, dysentery by TMPs of Santal tribe of Rangpur district [107]; helminthiasis, ulcer by Christians living in Mirzapur village of Dinajpur ditrict, Bangladesh [108]; helminthiasis, infections from scorpion bites by the Santal tribe residing in Thakurgaon district [111]; burning sensations within the chest, salty taste in mouth when burping, flatulency, gastric pain by TMPs of your Sigibe clan from the Khumi tribe of Thanchi sub-district in Bandarban district [115]. Coccinia cordifolia (L.) Cogn. Total paralysis or numbness of body, burning sensations in head or soles of feet by FMPs of two villages in Rajshahi district [38]; burning sensations for the duration of urination, diabetes by FMPs of Sylhet Division, Bangladesh [41]; burning sensations within the body, blood dysentery, scabies, leucoderma, diabetes in Shitol Para village, Jhalokati district [42]; coughs, diabetes, dysentery, emetic, burn by FMPs of 3 villages in Natore and Rajshahi districts [43]; mental disease, diabetes by FMPs of Daudkandi sub-district of Comilla district [44]; hypertension, diabetes by FMPs of Dinajpur district [45]; diabetes by FMPs of Feni district [47]; sunstroke, diabetes by FMPs in villages by the Bangali River of Bogra district [48]; headache by FMPs in villages by the Padma River of Rajshahi district [48]; diabetes, to help keep head cool, dysentery, skin ailments, burning sensations in hands or feet by FMPs of Station Purbo Para village, Jamalpur district [33]; hematemesis, loss of appetite, diabetes, flatulency by FMPs of Shetabganj village, Dinajpur district [32]; typhoid, eczema, leucoderma, lesion on tongue by FMPs of Daulatdia Ghat, Kushtia district [51]; diabetes, jaundice by FMPs of Vitbilia village in Pabna district [52]; diabetes, debility, to maintain head cool, burning sensations in the physique by FMPs of six villages in Greater Naogaon district [53]; dysentery, burns by FMPs of seven villages in Ishwardi Upazilla, Pabna district [54]; diabetes, stomach pain by FMPs of a village in Narayanganj district [55]; diarrhea, dysentery by a FMP of Gachabari village in Tangail district [56]; menstrual troubles like burning sensations during urination, frequent urination, diabetes by tribal medicinal practitioners (TMPs) of the Chakma tribe residing in Rangamati district [57]; diabetes, loss of appetite, flatulence by FMPs of 3 villages in Kurigram district [58]; burning sensations in the physique, diabetes by a FMP of Savar in Dhaka district [60]; moisturizer for dry skin by FMPs of four villages in Natore and Rajshahi districts [61]; blood purifier, loss of appetite, diabetes, injury, sprains by TMPs of.

รุ่นแก้ไขเมื่อ 12:44, 27 กันยายน 2564

Nd Shekhertek villages in Rangpur district [79]; discomfort in body, blood purifier Nd Shekhertek villages in Rangpur district [79]; discomfort in physique, blood purifier by FMPs of Terbaria and Babla villages in Tangail district [81]; pain by TMPs of Bongshi tribe in Tangail district [82]; malaria fever, any form of stomach discomfort by TMPs of Tonchongya tribe of Bandarban district [82]; feeling of weakness in the course of time of menstruation by TMPs on the Harbang clan from the Tripura tribe of Mirsharai area, Chittagong district [95]; fever in kids, toothache, discomfort in gums by FMPs of Dhamrai sub-district, Dhaka district [97]; hookworm infection by FMPs of Barisal Town, Barisal district [98]; stomach pain by TMPs of Chakma tribe of Rangapanir Chara Area in Khagrachaari district [34]; jaundice, helminthiasis by a TMP in the Deb barma clan on the Tripura tribe of Moulvibazar district [103]; jaundice by a FMP of Sreemangal Upazila in Maulvibazar district [105]; skin eruption, fever, dysentery by TMPs of Santal tribe of Rangpur district [107]; helminthiasis, ulcer by Christians living in Mirzapur village of Dinajpur ditrict, Bangladesh [108]; helminthiasis, infections from scorpion bites by the Santal tribe residing in Thakurgaon district [111]; burning sensations within the chest, salty taste in mouth when burping, flatulency, gastric pain by TMPs of your Sigibe clan from the Khumi tribe of Thanchi sub-district in Bandarban district [115]. Coccinia cordifolia (L.) Cogn. Total paralysis or numbness of body, burning sensations in head or soles of feet by FMPs of two villages in Rajshahi district [38]; burning sensations for the duration of urination, diabetes by FMPs of Sylhet Division, Bangladesh [41]; burning sensations within the body, blood dysentery, scabies, leucoderma, diabetes in Shitol Para village, Jhalokati district [42]; coughs, diabetes, dysentery, emetic, burn by FMPs of 3 villages in Natore and Rajshahi districts [43]; mental disease, diabetes by FMPs of Daudkandi sub-district of Comilla district [44]; hypertension, diabetes by FMPs of Dinajpur district [45]; diabetes by FMPs of Feni district [47]; sunstroke, diabetes by FMPs in villages by the Bangali River of Bogra district [48]; headache by FMPs in villages by the Padma River of Rajshahi district [48]; diabetes, to help keep head cool, dysentery, skin ailments, burning sensations in hands or feet by FMPs of Station Purbo Para village, Jamalpur district [33]; hematemesis, loss of appetite, diabetes, flatulency by FMPs of Shetabganj village, Dinajpur district [32]; typhoid, eczema, leucoderma, lesion on tongue by FMPs of Daulatdia Ghat, Kushtia district [51]; diabetes, jaundice by FMPs of Vitbilia village in Pabna district [52]; diabetes, debility, to maintain head cool, burning sensations in the physique by FMPs of six villages in Greater Naogaon district [53]; dysentery, burns by FMPs of seven villages in Ishwardi Upazilla, Pabna district [54]; diabetes, stomach pain by FMPs of a village in Narayanganj district [55]; diarrhea, dysentery by a FMP of Gachabari village in Tangail district [56]; menstrual troubles like burning sensations during urination, frequent urination, diabetes by tribal medicinal practitioners (TMPs) of the Chakma tribe residing in Rangamati district [57]; diabetes, loss of appetite, flatulence by FMPs of 3 villages in Kurigram district [58]; burning sensations in the physique, diabetes by a FMP of Savar in Dhaka district [60]; moisturizer for dry skin by FMPs of four villages in Natore and Rajshahi districts [61]; blood purifier, loss of appetite, diabetes, injury, sprains by TMPs of.

ดึงข้อมูลจาก "https://wiki.surinsanghasociety.com/index.php?title=หน้าหลัก&oldid=495923"