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Era and Recombinant Monoclonal Antibodies Purified human monoclonal recombinant NMO-IgGs (rAb Era and Recombinant Monoclonal Antibodies Purified human monoclonal recombinant NMO-IgGs (rAb)-53 and rAb-58 were generated as described (Bennett et al., 2009), with a measles virus-specific rAb (2B4) used as an isotype-matched control. Labeling of rAb-58 with the red fluorophore Cy3 (rAb-58-Cy3) was done using the Amersham Cy3 Ab Labelling Kit (GE Health-care, Buckinghamshire, UK). NMO serum from six seropositive NMO patients was provided by Accelerated Cure (SB-480848 Inhibitor Waltham, MA). Control (non-NMO) human serum was obtained from three non-NMO individuals. For some studies, total IgG was purified and concentrated from serum using a Melon Gel IgG Purification Kit (Thermo Fisher Scientific, Rockford, I.