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Hese assignments were supported with either CID or ETD data. While Hese assignments were supported with either CID or ETD data. While this glycan is unexpected, we consistently observed Nlinked HexNAc modification on the previous glycosylation analysis of a 293T cell-derived gp140 counterpart1 and it was also reported on the O-GlcNAcylation of postsynaptic density pseudo-organelle derived from murine brains45. To distinguish cell line dependent differences in the glycan profiles, glycan profile at each site were evaluated for high mannose or processed (hybrid and complex-type) glycan content using the criteria previously described1,37,39. The glycan profile at each site was compared using heirarchical cluster analysis to infer the degree of similarity betwe.