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Protonated system is difficult for mixing times m 100 ms, and for Protonated system is difficult for mixing times m 100 ms, and for systems whose global correlation times c > 10 ns (Supporting Information Figures S1 and S2). Thus reducing the proton density results in a more efficient and accurate identification of the interface over a wider range of molecular masses. An extensive network of cross-relaxing Title Loaded From File nuclei determines the longitudinal relaxation of protons in a macromolecule. Additional complexities may arise when one considers a REDPRO labeled protein. If a protein is deuterated to a level of 90 , then the probability of finding a proton in any particular site is only 10 . The multi-exponential relaxation behavior reflects an average over all poss.