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Red averages of the curves of y-value ratios across 3 data points Red averages of the curves of y-value ratios across 3 data points, between x=0nm and x=40nm) We refer to this measure as the extent of mixing between the two molecules of interest. This measure can be averaged for multiple cells and its values can be compared to the extent of mixing of other molecules or other cells under varying experimental conditions.NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author Manuscript 4. Results4.1. Protein clustering 4.1.1 Univariate analysis and protein clustering--The self-association of proteins at the PM occurs at two levels, protein complexes at the nanoscale and larger clusters or microclusters visible at the diffraction-limited light microsc.